CST, 4089S, Jak2 (D2E12) Rabbit Monoclonal Antibody (Sepharose® Bead Conjugate)

Monoclonal Antibody for studying Jak2. Validated for Immunoprecipitation. Highly specific and rigorously validated in-house, Jak2 (D2E12) Rabbit Monoclonal Antibody (Sepharose^® Bead Conjugate) (CST #4089) is ready to ship. Product Usage Information Immunoprecipitation: 1:20 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at -20°C. Do not aliquot the antibodies. Protocol Available protocols: Immunoprecipitation Specificity / Sensitivity Jak2 (D2E12) Rabbit Monoclonal Antibody (Sepharose Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunzing animals with a synthetic peptide corresponding to residues surrounding Pro841 of Jak2. Background Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. Alternate Names JAK-2; JAK2; Janus kinase 2; Janus kinase 2 (a protein tyrosine kinase); JTK10; Tyrosine-protein kinase JAK2 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 125 Source/Isotype: Rabbit IgG