CST, 40908SF, BCKDH-E1 alpha (E7Y1I) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying BCKDH E1-alpha. Validated for ELISA+. Highly specific and rigorously validated in-house, BCKDH-E1 alpha (E7Y1I) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #40908) is ready to ship. Product Usage Information This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity BCKDH-E1 alpha (E7Y1I) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total BCKDH-E1α protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to a fragment of human BCKDH-E1α protein. Background Branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (1). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (2,3). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (3). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex (3-5). Alternate Names 2-oxoisovalerate dehydrogenase (lipoamide); 2-oxoisovalerate dehydrogenase subunit alpha, mitochondrial; BCKDE1A; BCKDH E1-alpha; BCKDHA; branched chain keto acid dehydrogenase E1 alpha protein; branched chain keto acid dehydrogenase E1 subunit alpha; branched chain keto acid dehydrogenase E1, alpha polypeptide; Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain; FLJ45695; MSU; MSUD1; ODBA; OVD1A Specification REACTIVITY: H M R SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG