CST, 4092P, Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit Monoclonal Antibody (Biotinylated)

Monoclonal Antibody for studying p38-alpha MAPK (Thr180/Tyr182) phosphate/p38-delta MAPK (Thr180/Tyr182) phosphate/p38-gamma MAPK (Thr183/Tyr185) phosphate/p38-beta MAPK (Thr180/Tyr182) phosphate. Validated for Western Blotting,Peptide ELISA (DELFIA). Highly specific and rigorously validated in-house, Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit Monoclonal Antibody (Biotinylated) (CST #4092) is ready to ship. Product Usage Information Biotinylated antibodies are ideal for immunoassay technologies and high-throughput ELISA platforms that require antibody pairs where both antibodies are from the same host. Platforms utilizing biotinylated antibodies include, but are not limited to, MSD, xMAP, Quanterix Simoa, AlphaLISA, AlphaScreen, HTRF, LANCE, and TR-FRET. Optimal dilutions/working concentrations should be determined by the end user. Please contact us if you require the antibody clone biotinylated at a different concentration, a carrier-free formulation, or a more customized packaging solution. Storage Supplied in 140 mM NaCl, 3 mM KCl, 10 mM sodium phosphate (pH 7.4) dibasic, 2 mM potassium phosphate monobasic, 2 mg/mL BSA, and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-p38 MAPK (Thr180/Tyr182) (3D7) Rabbit Monoclonal Antibody (Biotinylated) detects endogenous levels of p38 MAPK only when phosphorylated at both Thr180 and Tyr182. This antibody does not cross-react with the phosphorylated forms of either p42/44 MAPK or SAPK/JNK. Species Reactivity: Human, Mouse, Rat, Monkey, D. melanogaster, Pig, S. cerevisiae Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr180/Tyr182 of human p38 MAPK. Background p38 MAP kinase (MAPK), also called RK (1) or CSBP (2), is the mammalian orthologue of the yeast HOG kinase that participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAPK, p38α, β, γ (also known as Erk6 or SAPK3), and δ (also known as SAPK4) have been identified. Similar to the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses, including osmotic shock, inflammatory cytokines, lipopolysaccharide (LPS), UV light, and growth factors (1-5). MKK3, MKK6, and SEK activate p38 MAPK by phosphorylation at Thr180 and Tyr182. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6), and MEF2 (5-8). SB203580 (4-(4-fluorophenyl)-2-(4-methylsulfinylphenyl)-5-(4-pyridyl)-imidazole) is a selective inhibitor of p38 MAPK. This compound inhibits the activation of MAPKAPK-2 by p38 MAPK and subsequent phosphorylation of HSP27 (9). SB203580 inhibits p38 MAPK catalytic activity by binding to the ATP-binding pocket, but does not inhibit phosphorylation of p38 MAPK by upstream kinases (10). Alternate Names CSAID-binding protein; Csaids binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; Cytokine suppressive anti-inflammatory drug-binding protein; ERK-6; ERK3; ERK6; EXIP; Extracellular signal-regulated kinase 6; MAP kinase 11; MAP kinase 12; MAP kinase 13; MAP kinase 14; MAP kinase MXI2; MAP kinase p38 alpha; MAP kinase p38 beta; MAP kinase p38 delta; MAP kinase p38 gamma; MAPK 11; MAPK 12; MAPK 13; MAPK 14; MAPK-13; MAPK11; MAPK12; MAPK13; MAPK14; MAX-interacting protein 2; MGC99536; Mitogen-activated protein kinase 11; Mitogen-activated protein kinase 12; Mitogen-activated protein kinase 13; Mitogen-activated protein kinase 14; mitogen-activated protein kinase 3; Mitogen-activated protein kinase p38 alpha; Mitogen-activated protein kinase p38 beta; Mitogen-activated protein kinase p38 delta; Mitogen-activated protein kinase p38 gamma; mitogen-activated protein kinase p38-2; MK11; MK12; MK13; MK14; MXI2; p38; p38 MAP kinase; p38 mitogen activated protein kinase; p38-2; p38-alpha; P38A; p38ALPHA; p38alpha Exip; p38b; p38Beta; P38BETA2; P38D; p38delta; P38G; P38GAMMA; PRKM11; PRKM12; PRKM13; PRKM14; PRKM15; RK; SAPK-3; SAPK2; SAPK2A; SAPK2B; SAPK3; SAPK4; Stress-activated protein kinase 2; stress-activated protein kinase 2A; Stress-activated protein kinase 2b; Stress-activated protein kinase 3; Stress-activated protein kinase 4; stress-activated protein kinase-2; stress-activated protein kinase-2b Specification REACTIVITY: H M R Mk Dm Pg Sc SENSITIVITY: Endogenous MW (kDa): 43 Source/Isotype: Rabbit IgG