CST, 40941S, IGF-II (E3F3N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IGF2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, IGF-II (E3F3N) Rabbit Monoclonal Antibody (CST #40941) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity IGF-II (E3F3N) Rabbit Monoclonal Antibody recognizes endogenous levels of total IGF-II protein. This antibody does not cross-react with IGF-I protein. This antibody also cross-reacts with multiple non-specific bands at higher molecular weights with unknown origin. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Cys84 of human IGF-II protein. Background Insulin-like growth factor 2 (IGF-II, IGF-2) is a peptide growth factor with structural similarity to insulin and insulin-like growth factor 1 (IGF-I). IGF-II is reported to interact with multiple cell-surface receptors, including type 1 insulin-like growth factor receptor (IGF1R), insulin receptor A isoform (IR-A), and the structurally distinct type-2 IGF receptor (IGF2R) (1,2). Activation of IGF1R and IR-A by IGF-II promotes cell growth, proliferation, apoptosis, and metabolic signaling (3,4); binding of IGF-II to IGF2R (a.k.a. mannose 6-phosphate receptor) results in IGF-II internalization via receptor-mediated endocytosis, and subsequent transport to the lysosome for degradation (5). Serum IGF-II is generally found in complex with insulin-like growth factor binding proteins (IGFBPs), to which it binds with high affinity; this interaction functions to prolong the half-life of IGF-II in circulation (3). IGF-II plays a particularly crucial role in promoting embryonic fetal growth and development (6-9); in rodents, many of these functions are displaced by IGF-I during normal postnatal development, whereas in humans, IGF-II expression is more widely maintained (10). Binding of IGF-II to both IGF1R and IR-A has been shown to promote cancer progression, primarily through promoting cell proliferation and survival (11,12). Small molecules designed to block IGF-II activity are thus being pursued as therapeutic strategies for cancer treatment (13). Alternate Names C11orf43; FLJ22066; FLJ44734; GRDF; IGF-II; IGF2; INSIGF; insulin like growth factor 2; insulin-like growth factor 2; insulin-like growth factor 2 (somatomedin A); Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; insulin-like growth factor type 2; pp9974; Preptin; putative insulin-like growth factor II associated protein; somatomedin A; Somatomedin-A; SRS3; T3M-11-derived growth factor Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 9, 15, 17, 19, 23 Source/Isotype: Rabbit IgG