CST, 41019S, MAFB Antibody

Polyclonal Antibody for studying MAFB. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, MAFB Antibody (CST #41019) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MAFB Antibody recognizes endogenous levels of total MAFB protein. Based on sequence similarity, this antibody is not predicted to cross-react with MAFA. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding of human Pro188 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background MAFB belongs to the musculoaponeurotic fibrosarcoma (MAF) family of basic leucine-zipper transcription factors (1). In mouse embryo, MAFB expression is first detected at E10.5 (2,3). Early in development, MAFB drives differentiation of both glucagon-producing α-cells and insulin-producing β-cells in the pancreas, but later plays a more decisive role in the maturation and maintenance of functional α-cells (4,5). Consistent with MAFB playing a critical role in mature α-cells, MAFB is enriched in α-cells within two weeks of birth in the pancreas (6). Glucagon and insulin secretion is tightly regulated, and imbalances in these hormones contribute to metabolic conditions. Therefore, understanding the role of MAFB in α-cell development, maintenance, and physiological function may contribute to developing deeper insights into how these cells contribute to metabolic diseases like diabetes. MAFB also regulates monocyte differentiation, indicating MAFB functions beyond the pancreas (7). Alternate Names DURS3; Kreisler maf-related leucine zipper homolog; KRML; MAF bZIP transcription factor B; Maf-B; MAFB; MAFB/Kreisler basic region/leucine zipper transcription factor; MCTO; MGC43127; Transcription factor MafB; v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog B; V-maf musculoaponeurotic fibrosarcoma oncogene homolog B; v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 46, 48 SOURCE: Rabbit