CST, 4107S, HSL Antibody

Polyclonal Antibody for studying HSL. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HSL Antibody (CST #4107) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HSL Antibody detects endogenous levels of total HSL protein. Species Reactivity: Human, Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly944 of human HSL protein. Antibodies are purified by peptide affinity chromatography. Background HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4). Alternate Names AOMS4; FPLD6; Hormone-sensitive lipase; hormone-sensitive lipase testicular isoform; HSL; LHS; lipase E, hormone sensitive type; lipase, hormone-sensitive; LIPE; LIPS; Monoacylglycerol lipase LIPE; REH; Retinyl ester hydrolase Specification REACTIVITY: H M SENSITIVITY: Endogenous MW (kDa): 81, 83 SOURCE: Rabbit