CST, 41081S, INF2 (E7H5D) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying INF2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, INF2 (E7H5D) Rabbit Monoclonal Antibody (CST #41081) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity INF2 (E7H5D) Rabbit Monoclonal Antibody recognizes endogenous levels of total INF2 protein. INF2 isoforms 1 and 2 appear as a doublet in the 170 kDa range using western blot analysis. This antibody also detects a band at approximately 100 kDa of unknown origin. This band may be a carboxy-terminal cleavage product of INF2. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro1019 of human INF2 protein. Background Inverted formin 2 (INF2) is a member of the formin family of actin assembly factors. INF2 regulates multiple cellular processes, including mitochondrial fission and vesicle trafficking. INF2 also regulates microtubule stability in conjunction with the formin mDia1 and IQGAP1 (1). Mutations in INF2 have been linked to kidney disease and neuropathy in humans (2). INF2 stimulates Drp1-mediated and Drp1-independent mitochondrial division (3). Speckle-type POZ Protein (SPOP), a potential driver in human prostate cancer, regulates INF2 activity through ubiquitination, affecting INF2-mediated migration, invasion, and mitochondrial fission in prostate cancer (4). In thyroid cancer, JNK2 signaling mediates INF2-related mitochondrial fission to promote apoptosis (5). Alternate Names C14orf151; C14orf173; CMTDIE; DKFZp762A0214; FLJ22056; FSGS5; HBEAG-binding protein 2 binding protein C; HBEBP2-binding protein C; INF2; inverted formin 2; Inverted formin-2; inverted formin, FH2 and WH2 domain containing; MGC13251; pp9484 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 170 Source/Isotype: Rabbit IgG