CST, 4112S, Cyclin B1 (V152) Mouse Monoclonal Antibody (Alexa Fluor® 488 Conjugate)

Monoclonal Antibody for studying Cyclin B1. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, Cyclin B1 (V152) Mouse Monoclonal Antibody (Alexa Fluor^® 488 Conjugate) (CST #4112) is ready to ship. Product Usage Information Flow Cytometry (Fixed/Permeabilized): 1:50 Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity Cyclin B1 (V152) Mouse Monoclonal Antibody (Alexa Fluor Species Reactivity: Human, Mouse Source / Purification Cyclin B1 (V152) Monoclonal antibody is produced by immunizing animals with a peptide corresponding to a sequence from hamster cyclin B1. The antibody was conjugated to Alexa Fluor 488 under optimal conditions with an F/P ratio of 2-5. Background Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) (1,2) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C (3-5). Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis (6-9). While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147 (6,10). At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression (11). Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers (12-14). Alternate Names CCNB; CCNB1; cyclin B1; G2/mitotic-specific cyclin B1; G2/mitotic-specific cyclin-B1 Specification REACTIVITY: H M SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1