CST, 41169T, SF3A1 (F8X7A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SF3A1. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, SF3A1 (F8X7A) Rabbit Monoclonal Antibody (CST #41169) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SF3A1 (F8X7A) Rabbit Monoclonal Antibody recognizes endogenous levels of total SF3A1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro537 of human SF3A1 protein. Background The mammalian splicing factor SF3a contains three primary subunits: SF3A1 (SF3a120), SF3A2 (SF3a66), and SF3A3 (SF3a60), which, along with SF3b, activate the U2 snRNP to form the pre-mRNA splicing complex (1,2). SF3A2 and SF3A3 interact with SF3A1, but not with each other, and SF3A3 plays a major role in recruiting SF3A1 to the complex (3). Each protein in the heterotrimer has been shown to be essential for mRNA splicing and cell viability (4). Notably, SF3a plays a role in growth factor signaling as SF3A1 can bind to ERα when phosphorylated at Ser118 to affect downstream splicing (5). SF3a also plays a role in the innate immune response, promoting inflammation by splicing the TLR adaptor Myd88. Furthermore, SF3a regulates several genes involved in innate immune signaling pathways in macrophages versus other pathways (6,7). Alternate Names pre-mRNA processing 21; pre-mRNA splicing factor SF3a (120 kDa subunit); PRP21; PRPF21; SAP 114; SAP114; SF3A1; SF3a120; spliceosome associated protein 114; Spliceosome-associated protein 114; splicing factor 3 subunit 1; Splicing factor 3A subunit 1; splicing factor 3a, subunit 1, 120kD; splicing factor 3a, subunit 1, 120kDa Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 95, 120 Source/Isotype: Rabbit IgG