CST, 41182S, WWP2 (E8W8A) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying WWP2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, WWP2 (E8W8A) Rabbit Monoclonal Antibody (CST #41182) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity WWP2 (E8W8A) Rabbit Monoclonal Antibody recognizes endogenous levels of total WWP2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val349 of human WWP2 protein. Background Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Ubiquitin is first activated by forming a thiolester complex with the activation component E1. The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-NH of the target protein lysine residue (1-3). Research studies suggest that activated E2 associates transiently with E3, and the dissociation is a critical step for ubiquitination (4). WWP2 (WW domain-containing protein 2, AIP2) is a member of the NEDD4 family of E3 ligases, which play an important role in protein ubiquitination. WWP2 may play a role in multiple processes, including chondrogenesis and oncogenic signaling pathway regulation via interactions with Smad proteins and the tumor suppressor PTEN. It has recently become clear that aberrant activity of WWP2 may play a role in multiple tumor types and promotes tumorigenesis through the PTEN/Akt signaling pathway. Recently, accumulating evidence indicates that WWP2 plays a crucial part in the pathogenesis of different tumor types (5). Alternate Names AIP2; atrophin-1 interacting protein 2; Atrophin-1-interacting protein 2; HECT-type E3 ubiquitin transferase WWP2; Nedd-4-like ubiquitin-protein ligase; NEDD4-like E3 ubiquitin-protein ligase WWP2; WW domain containing E3 ubiquitin protein ligase 2; WW domain-containing protein 2; WWP2; WWp2-like Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG