CST, 4120S, Microcephalin-1/BRIT1 (D38G5) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MCPH1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Microcephalin-1/BRIT1 (D38G5) Rabbit Monoclonal Antibody (CST #4120) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Microcephalin-1/BRIT1 (D38G5) Rabbit Monoclonal Antibody detects endogenous levels of total Microcephalin-1/BRIT1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human Microcephalin-1/BRIT1. Background Microcephalin-1 (MCPH1)/BRIT1 is an early DNA damage response protein named for its mutated state in the human disease primary microcephaly. BRIT1 forms damage-induced nuclear foci, is involved in DNA damage and cell cycle checkpoints as well as regulation of mitosis. BRIT1 function is necessary for DNA damage responses, and the absence of BRIT1 function leads to genome instability. A potential tumor suppressor, BRIT1 expression is reduced in human carcinomas (1-2, reviewed in 3). BRIT1 colocalizes with other DNA repair proteins (53BP1, MDC1, NBS1, ATM, RPA, and ATR) and is required for their activation (2). BRIT1 likely regulates DNA repair through chromatin remodeling in response to DNA damage, allowing access of repair proteins to DNA (4). Alternate Names BRCT-repeat inhibitor of TERT expression 1; BRIT1; FLJ12847; MCPH1; MCT; Microcephalin; microcephalin 1; truncated microcephalin Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Rabbit IgG