CST, 41220S, IL-27RA (E3L6U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying IL27RA. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, IL-27RA (E3L6U) Rabbit Monoclonal Antibody (CST #41220) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity IL-27RA (E3L6U) Rabbit Monoclonal Antibody recognizes endogenous levels of total IL-27RA protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL-27RA protein. Background Interleukin 27 receptor α (IL-27RA or IL-27Rα), also known as T cell cytokine receptor (TCCR) and WSX-1, forms a receptor complex with gp130 to bind and enable IL-27 signaling (1,2). IL-27Rα is expressed on T cells, B cells, natural killer (NK) cells, monocytes, macrophages, dendritic cells (DCs), as well as on subsets of epithelial and endothelial cells (3). Binding of IL-27 to its receptor complex induces the activation of receptor-associated Jak1 and Jak2, which in turn phosphorylates Stat1 and Stat3 (4,5). IL-27 signaling plays an important anti-inflammatory role in chronic immune-mediated disease and infectious disease, and enhances the expression of co-inhibitory molecules LAG-3, TIM-3, PD-1, and TIGIT (6-8). Due to its immunosuppressive role in cancers, IL-27 has been identified as a target for cancer immunotherapy (9). Alternate Names class I cytokine receptor; CRL1; Cytokine receptor WSX-1; Cytokine receptor-like 1; I27RA; IL-27 receptor subunit alpha; IL-27R subunit alpha; IL-27R-alpha; IL-27RA; IL27R; IL27RA; interleukin 27 receptor subunit alpha; interleukin 27 receptor, alpha; Interleukin-27 receptor subunit alpha; T-cell cytokine receptor type 1; TCCR; Type I T-cell cytokine receptor; WSX-1; WSX1; ZcytoR1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 95, 105 Source/Isotype: Rabbit IgG