CST, 41222S, GADD34 (E3S6N) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying GADD34. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, GADD34 (E3S6N) Rabbit Monoclonal Antibody (CST #41222) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity GADD34 (E3S6N) Rabbit Monoclonal Antibody recognizes endogenous levels of total GADD34 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala69 of human GADD34 protein. Background The growth arrest and DNA damage-inducible protein, GADD34, is essential for cell stress responses. It is induced by stresses such as DNA damage and endoplasmic reticulum (ER) stress. ER stress stimulates the unfolded protein response (UPR), increasing phosphorylation of Ser51 in the α subunit of eukaryotic translation initiation factor 2 (eIF2α). This change represses translation. Phosphorylation of eIF2α also increases GADD34 expression. GADD34 binds to type 1 protein phosphatase 1 (PP1), a serine/threonine phosphatase, and promotes PP1 to dephosphorylate eIF2α (1,2). GADD34 expression is correlated with eIF2α dephosphorylation late in the stress response, suggesting a critical role of GADD34 in a negative feedback loop to reduce stress response signaling and shift cells from protein synthesis inhibition to stress-induced gene expression (3). Alternate Names GADD34; Growth arrest and DNA damage-inducible protein GADD34; growth arrest and DNA-damage-inducible 34; Myeloid differentiation primary response protein MyD116 homolog; PPP1R15A; PR15A; Protein phosphatase 1 regulatory subunit 15A; protein phosphatase 1, regulatory (inhibitor) subunit 15A Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 110 Source/Isotype: Rabbit IgG