CST, 41228S, HLA-F (F4B9F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HLA-F. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, HLA-F (F4B9F) Rabbit Monoclonal Antibody (CST #41228) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity HLA-F (F4B9F) Rabbit Monoclonal Antibody recognizes endogenous levels of total HLA-F protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln162 of human HLA-F protein. Background HLA class I histocompatibility antigen, alpha chain F (HLA-F) is a nonclassical member of the HLA class I family and has less genetic diversity than classical HLA molecules (1-4). HLA-F expression is primarily intracellular but has been observed on the surface in some contexts, including some activated immune cell subsets, virus-infected cells, invasive extravillous cytotrophoblasts, and various cancer types (5,6). HLA-F can be expressed on the surface as an open conformer monomer or dimer, or in a complex with β2-microglobulin and peptide. As an open conformer monomer, HLA-F is observed to interact with activating KIR3DS1 and inhibitory KIR3DL2 (7,8). The HLA-F/β2-microglobulin peptide complex interacts with inhibitory receptors ILT2 and ILT4 (9,10). It is hypothesized that high surface expression of HLA-F in tumors can be a mechanism of immune escape through interaction with inhibitory receptors on immune cells (5). In the context of viral infection, HLA-F interaction with activating KIR3DS1 on natural killer (NK) cells can result in NK cell degranulation and cytokine production (7). Alternate Names CDA12; HLA class I histocompatibility antigen, alpha chain F; HLA class I molecule; HLA F antigen; HLA-5.4; HLA-CDA12; HLA-F; HLAF; Leukocyte antigen F; major histocompatibility complex, class I, F; MHC class I antigen; MHC class I antigen F; MHC class Ib antigen Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 42 Source/Isotype: Rabbit IgG