CST, 41248SF, Rab7 (D95F2) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Rab7. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Rab7 (D95F2) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #41248) is ready to ship. Product Usage Information This product is the carrier free version of product #9367. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 9367 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity Rab7 (D95F2) Rabbit Monoclonal Antibody (BSA and Azide Free) detects endogenous levels of total Rab7 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu188 of human Rab7 protein. Background Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosomes and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10). Alternate Names CMT2B; FLJ20819; PRO2706; RAB7; RAB7, member RAS oncogene family; RAB7A; RAB7A, member RAS oncogene family; Ras-associated protein RAB7; Ras-related protein Rab-7a Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 23 Source/Isotype: Rabbit IgG