CST, 41255S, SLK Antibody

Polyclonal Antibody for studying SLK. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, SLK Antibody (CST #41255) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity SLK Antibody recognizes endogenous levels of total SLK protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu431 of human SLK protein. Background SLK (Ste20-like Kinase) is a member of the germinal center kinase (GCK) family of proteins. SLK has a kinase domain located at the N terminus (1). The autophosphorylation of SLK at Thr183 and Ser189 is required for the upregulation of SLK kinase activity (1, 2). The protein also has a caspase cleavage site DXXD and a SH3 binding site PXXP located in the middle part of its sequence, and a regulatory C terminal coiled-coil domain for homodimerization and adaptor binding (1-4). SLK plays important roles in development, tissue regeneration and cancer cell migration by regulating several signaling pathways (5-7). SLK phosphorylates and activates ASK1 to induce downstream p38 phosphorylation and apoptosis (8,9). During cell cycle, SLK phosphorylates Polo-like kinase (PLK) at Thr210 to promote G2/M transition (10,11). SLK also promotes cell division by direct phosphorylation of ERMs and dynactin to activate microtubule reorganization and spindle orientation (12, 13). During focal adhesion and cell migration process, SLK is activated and colocalized to the focal adhesion complex where it promotes complex turnover by phosphorylating paxillin at Ser250 (14, 15). Alternate Names bA16H23.1; CTCL tumor antigen se20-9; hSLK; KIAA0204; Long Ste20-like Kinase; LOSK; MGC133067; se20-9; Serine/threonine-protein kinase 2; SLK; SNF1 (sucrose nonfermenting, yeast, homolog)-like kinase, SNF1 sucrose nonfermenting like kinase; SNF1 sucrose nonfermenting like kinase; STE20 like kinase; STE20-like kinase; STE20-like kinase (yeast); STE20-like serine/threonine-protein kinase; STE20-related kinase; Ste20-related serine/threonine kinase; STE20-related serine/threonine-protein kinase; STK2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 220 SOURCE: Rabbit