CST, 4125S, TLK1 Antibody

Polyclonal Antibody for studying TLK1. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, TLK1 Antibody (CST #4125) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity TLK1 Antibody detects endogenous levels of total TLK1. This antibody may cross-react with TLK2. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues adjacent to Ser183 of human TLK1. Antibodies are purified by protein A and peptide affinity chromatography. Background Tousled-like kinases (TLK1 and TLK2) are nuclear serine/threonine kinases named for their homology to the Tousled gene from Arabidopsis thaliana, essential for flower development (1). The kinase activities of the TLKs are cell cycle regulated, with maximal activity during S phase (1). TLK appears to play a role in chromatin assembly and DNA damage checkpoint regulation (1,2). In C. elegans, TLK1 is essential for appropriate transcription during embryonic development (3). Substrates for TLK include the human chromatin assembly factor Asf, which functions in DNA replication- and repair-coupled chromatin assembly (2). DNA damage during S phase, when TLK is maximally active, leads to inhibition of TLK activity (1). This inhibition requires ataxia mutated kinase (ATM) and Chk1 (4,5). ATM and the related kinase ATR are activited by DNA damage during S phase, phosphorylate Chk1/Chk2, and block the transition into mitosis (6). Chk1 phosphorylates TLK1 on Ser743 in vitro and in vivo, leading to inhibition of TLK1 activity (4). This process likely provides a mechanism to slow the chromatin assembly processes controlled by TLK in the event of DNA damage. Alternate Names KIAA0137; PKU-beta; serine threonine protein kinase; Serine/threonine-protein kinase tousled-like 1; SNARE protein kinase SNAK; TLK1; TLK1 iso2; tousled like kinase 1; Tousled-like kinase 1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 86 SOURCE: Rabbit