CST, 41262T, Siglec-10 (D4E3Z) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Siglec-10 mouse. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, Siglec-10 (D4E3Z) Rabbit Monoclonal Antibody (CST #41262) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Siglec-10 (D4E3Z) Rabbit Monoclonal Antibody recognizes endogenous levels of total Siglec-10 protein. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro250 of mouse Siglec-10 protein. Background Siglec-10 is a sialic acid-binding Ig-like lectin that has been shown to negatively regulate innate and adaptive immune system function (1). Siglec-10 is a type 1 transmembrane protein that has four extracellular Ig-like domains, a transmembrane region, and a cytoplasmic tail with two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) (2). Siglec-10 is expressed in dendritic cells (DCs), monocytes, B cells, NK cells, and T cells (2,3). Siglec-10 interacts with CD24 to suppress immune responses to danger associated molecular patterns (DAMPs) by associating with the tyrosine phosphatase SHP-1, a negative regulator of nuclear factor-kappa B (NF-κB) (4). Siglec-10 has been shown to bind soluble CD52 leading to the impairment of phosphorylation of the T cell receptor-associated kinases Lck and Zap70 and T cell activation (3). It has been proposed that this mechanism of suppression could be involved in T cell homeostasis and the prevention of type I diabetes (3). Siglec-10 has also been identified as the leukocyte ligand for vascular adhesion protein-1 (VAP-1), which plays a key role in leukocyte trafficking (5). Lectin galactoside-binding soluble 3 binding protein (LGALS3BP), a tumor-associated immunomodulatory ligand, has also been shown to bind Siglec-10 (6). Alternate Names 9830164H23; A630096C01Rik; mSigle; mSiglec-G; sialic acid binding Ig-like lectin 10; sialic acid binding Ig-like lectin G; Sialic acid-binding Ig-like lectin 10; Sialic acid-binding Ig-like lectin G; SIG10; Siglec-10; Siglec-G; Siglec10; Siglecg Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 92, 100-105 Source/Isotype: Rabbit IgG