CST, 4129S, Cyclin E1 (HE12) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Cyclin E1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Cyclin E1 (HE12) Mouse Monoclonal Antibody (CST #4129) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85718 . Protocol Available protocols: Western Blotting Specificity / Sensitivity Cyclin E1 (HE12) Mouse Monoclonal Antibody detects endogenous levels of total cyclin E1 protein. This antibody does not cross react with cyclin E2. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant human cyclin E1 protein. The epitope has been mapped to residues surrounding Ile360. Background Cyclin E1 and cyclin E2 can associate with and activate CDK2 (1). Upon DNA damage, upregulation/activation of the CDK inhibitors p21 Waf1/Cip1 and p27 Kip1 prevent cyclin E/CDK2 activation, resulting in G1/S arrest. When conditions are favorable for cell cycle progression, cyclin D/CDK4/6 phosphorylates Rb and is thought to reduce the activity of p21 Waf1/Cip1 and p27 Kip1, allowing subsequent activation of cyclin E/CDK2 (1,2). Cyclin E/CDK2 further phosphorylates Rb to allow progression into S-phase, where cyclin E/CDK2 is thought to phosphorylate and activate multiple proteins involved in DNA synthesis (2,3). Turnover of cyclin E is largely controlled by phosphorylation that results in SCFFbw7-mediated ubiquitination and proteasome-dependent degradation (4,5). Cyclin E1 is phosphorylated at multiple sites including Thr62, Ser88, Ser72, Thr380, and Ser384, and is controlled by at least two kinases, CDK2 and GSK-3 (6,7). Alternate Names CCNE; CCNE1; cyclin E1; cyclin Es; cyclin Et; G1/S-specific cyclin-E1; pCCNE1 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Mouse IgG1