CST, 41302T, ESAM (F2Y5Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying ESAM. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, ESAM (F2Y5Q) Rabbit Monoclonal Antibody (CST #41302) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:300 - 1:1200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity ESAM (F2Y5Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total ESAM protein. This antibody detects a 75 kDa protein of unknown identity in some cell lines. Non-specific staining was observed in limited peripheral nerves, and in cardiac and skeletal muscle by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp351 of human ESAM protein. Background Endothelial cell-selective adhesion molecule (ESAM) is an immunoglobulin-like transmembrane protein expressed by endothelial cells and activated platelets (1-4). ESAM is associated with supporting the structural integrity of endothelial cell tight junctions (1-4). ESAM also plays a role in immune responses, aiding in the adhesion of leukocytes to the endothelial cells of blood vessels (4). In mice, expression levels of ESAM are used to differentiate subsets of dendritic cells (5,6). Alternate Names 2310008D05Rik; endothelial cell adhesion molecule; Endothelial cell-selective adhesion molecule; ESAM; HUEL (C4orf1)-interacting protein; LP4791 protein; W117m Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-65 Source/Isotype: Rabbit IgG