CST, 4134S, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Stat4 (Tyr693) phosphate. Validated for WB,IP,IF,F,ChIP. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody (CST #4134) is ready to ship. Product Usage Information For optimal ChIP results, use 10 μl of antibody and 10 μg of chromatin (approximately 4 x 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Immunoprecipitation: 1:100 Immunofluorescence (Immunocytochemistry): 1:1600 - 1:3200 Flow Cytometry (Fixed/Permeabilized): 1:1600 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 42353 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry), Flow Cytometry (Fixed/Permeabilized), Chromatin IP Specificity / Sensitivity Phospho-Stat4 (Tyr693) (D2E4) Rabbit Monoclonal Antibody recognizes endogenous levels of Stat4 protein only when phosphorylated at Tyr693. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr693 of human Stat4 protein. Background The Jak-Stat signaling pathway is utilized by a large number of cytokines, growth factors, and hormones (1). Receptor-mediated tyrosine phosphorylation of Jak family members triggers phosphorylation of Stat proteins, resulting in their nuclear translocation, binding to specific DNA elements, and subsequent activation of transcription. The remarkable range and specificity of responses regulated by the Stats is determined, in part, by the tissue-specific expression of different cytokine receptors, Jaks, and Stats, as well as by the combinatorial coupling of various Stat members to different receptors (2). Stat4 is predominantly expressed in the spleen, thymus, and testis and has been most extensively investigated as the mediator of IL-12 responses (3-8). Activation of Stat4 is associated with phosphorylation at Tyr693 (9). Stat4 is activated in response to IL-2 in natural killer (NK) cells, but not in T cells (10). Alternate Names Signal transducer and activator of transcription 4; signal transducer and activator of transcription 4 variant 3; SLEB11; STAT4 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 81 Source/Isotype: Rabbit IgG