CST, 41377T, MTHFD2 (D8W9U) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying MTHFD2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, MTHFD2 (D8W9U) Rabbit Monoclonal Antibody (CST #41377) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity MTHFD2 (D8W9U) Rabbit Monoclonal Antibody recognizes endogenous levels of total MTHFD2 protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu340 of human MTHFD2 protein. Background MTHFD2 is a bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase involved in mitochondrial folate metabolism (1). MTHFD2 expression is developmentally regulated, as it is expressed in embryos but not in most adult tissues. Recent research studies have shown that MTHFD2 is consistently overexpressed in many cancer types and correlated with poor survival in breast cancer (2-5). Overexpression of MTHFD2 promotes cell proliferation while its depletion induces cell death in human cancer cells (6). Alternate Names Bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial; Methenyltetrahydrofolate cyclohydrolase; methylene tetrahydrofolate dehydrogenase (NAD+ dependent), methenyltetrahydrofolate cyclohydrolase; methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase; MTDC; MTHFD2; NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase; NAD-dependent methylenetetrahydrofolate dehydrogenase; NMDMC Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG