CST, 4137S, Phospho-HSL (Ser565) Antibody

Polyclonal Antibody for studying HSL (Ser855) phosphate. Validated for Western Blotting,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-HSL (Ser565) Antibody (CST #4137) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Immunocytochemistry): 1:250 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-HSL (Ser565) Antibody detects endogenous levels of HSL only when phosphorylated at Ser565 by AMPK. This antibody does not cross-react with Ser563 phosphorylated HSL. Species Reactivity: Mouse Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser554 of human HSL (Ser565 of rat HSL). Antibodies are purified by protein A and peptide affinity chromatography. Background HSL (hormone-sensitive lipase) catalyzes the hydrolysis of triacylglycerol, the rate-limiting step in lipolysis. Lipolytic stimuli activate adenylyl cyclase and thus increase intracellular cAMP levels, which in turn activate protein kinase A (PKA). PKA phosphorylates HSL at Ser563, Ser659, and Ser660, which stimulates HSL activity (1,2). In contrast, AMPK phosphorylates HSL at Ser565, which reduces HSL phosphorylation at Ser563 by PKA and inhibits HSL activity (2,3). Recent work indicates that phosphorylation at Ser600 by p44/42 MAPKs also enhances the enzymatic activity of HSL (4). Alternate Names AOMS4; FPLD6; Hormone-sensitive lipase; hormone-sensitive lipase testicular isoform; HSL; LHS; lipase E, hormone sensitive type; lipase, hormone-sensitive; LIPE; LIPS; Monoacylglycerol lipase LIPE; REH; Retinyl ester hydrolase Specification REACTIVITY: M SENSITIVITY: Endogenous MW (kDa): 81, 83 SOURCE: Rabbit