CST, 41385S, eIF4A2 (F3S3X) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying eIF4A2. Validated for Western Blotting,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, eIF4A2 (F3S3X) Rabbit Monoclonal Antibody (CST #41385) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity eIF4A2 (F3S3X) Rabbit Monoclonal Antibody recognizes endogenous levels of total eIF4A2 protein. This antibody does not cross-react with eIF4A1 or eIF4A3 proteins. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His12 of human eIF4A2 protein. Background A variety of factors contribute to the important biological event of translation initiation. The eukaryotic initiation factor 4F (eIF4F) complex of translation initiation factors binds to the 5' m7 GTP cap to open up the mRNA secondary structure and allow small ribosome subunit binding (1). eIF4A, an eIF4 complex component that acts as an ATP-dependent RNA helicase, unwinds the secondary structure of the 5' mRNA untranslated region to mediate ribosome binding (2,3). In addition, eIF4A has recently been shown to repress Dpp/BMP signaling in a translation-independent manner in (4,5). eIF4A members (eIF4A1/2/3) are highly homologous proteins with non-redundant functions. eIF4A1 and eIF4A2 function within the cytoplasm, and their expression is correlated, whereas eIF4A3 functions in the nucleus as part of the exon junction complex (EJC) (6,7). eIF4A2 activity is critical in the functional gene regulation of microRNAs (8). eIF4A2 is capable of inhibiting deadenylation of mRNAs via CNOT7 inhibition (9). During stress response, eIF4A2 is sequestered into granules through Lys226 sumoylation (10). eIF4A2 plays an active role in promoting viral life cycle (11,12). Higher expression of eIF4A2 has been shown in different cancer types including esophageal squamous cell carcinoma and colorectal cancer (13,14). Alternate Names ATP-dependent RNA helicase eIF4A-2; BM-010; DDX2B; eIF-4A-II; EIF4A; eIF4A-II; EIF4A2; EIF4F; Eukaryotic initiation factor 4A-II; eukaryotic translation initiation factor 4A, isoform 2; eukaryotic translation initiation factor 4A2; IF4A2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 48 Source/Isotype: Rabbit IgG