CST, 41390S, CT45 (E2S3Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying CT45-1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, CT45 (E2S3Q) Rabbit Monoclonal Antibody (CST #41390) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity CT45 (E2S3Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total CT45-1 protein. This antibody is predicted to cross-react with all CT45 family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro93 of human CT45-1 protein. Background Cancer/testis antigens (CTAs) are a family of more than 100 proteins whose normal expression is largely restricted to immune-privileged germ cells of the testis, ovary, and trophoblast cells of the placenta. Although most normal somatic tissues are void of CTA expression, due to epigenetic silencing of gene expression, their expression is upregulated in a wide variety of human solid and liquid tumors (1,2). As such, CTAs have garnered much attention as attractive targets for a variety of immunotherapy-based approaches to selectively attack tumors (3). The CT45 family is a tandem cluster of highly similar genes that share many features with other classic CT genes, including Xq localization, multigene family, and identical or near-identical gene copies, indicating recent gene duplications (4). The nuclear protein antigen CT45 is often highly expressed in lung cancer and ovarian cancer, and the frequency and characteristics of CT45 expression are similar to those of other CT cancer vaccine targets currently in clinical trials, including NY-ESO-1 and MAGE-A (5). Overexpression of CT45A1 in breast cancer cells was found to upregulate various oncogenic and metastatic genes, promote epithelial-mesenchymal transition, and increase cell stemness, tumorigenesis, and metastasis (6). Additionally, research studies have shown that CT45A1 silencing suppresses cell proliferation and metastasis in breast and lung cancer cells by downregulating the ERK/CREB signaling pathway (6,7). Therefore, CT45A1 and other CT45 family members are excellent targets for anticancer drug discovery and targeted tumor therapy. Alternate Names Cancer/testis antigen 45-1; Cancer/testis antigen 45A1; cancer/testis antigen CT45-1; Cancer/testis antigen family 45 member A1; cancer/testis antigen family 45, member A1; CT45; CT45-1; CT45.1; CT451; CT45A1 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 26 Source/Isotype: Rabbit IgG