CST, 4140S, Phospho-Numb (Ser276) Antibody

Polyclonal Antibody for studying Numb (Ser276) phosphate. Validated for Western Blotting. Highly specific and rigorously validated in-house, Phospho-Numb (Ser276) Antibody (CST #4140) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Phospho-Numb (Ser276) Antibody detects endogenous levels of Numb protein only when phosphorylated on Ser276. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to Ser276 of the human Numb protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Numb contains an amino-terminal phosphotyrosine binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G protein-coupled receptor (GPCR) and protein kinase C (PKC) signaling (9). Numb can be phosphorylated at several sites including Ser7, Ser276 and Ser295. Phosphorylation at these sites regulates asymmetric membrane localization of Numb and integrin endocytosis (10-12). Alternate Names c14_5527; C14orf41; h-Numb; NUMB; NUMB endocytic adaptor protein; numb homolog; numb homolog (Drosophila); Protein numb homolog; Protein S171; S171 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 72, 74 SOURCE: Rabbit