CST, 41440S, OAS3 Antibody

Polyclonal Antibody for studying OAS3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, OAS3 Antibody (CST #41440) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:100 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity OAS3 Antibody recognizes endogenous levels of total OAS3 protein. This antibody detects an 18 kDa band of unknown origin in some cell lines. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human OAS3 protein. Antibodies are purified by peptide affinity chromatography. Background The 2'-5'-oligoadenylate synthetase (OAS) family of interferon-induced proteins play an important role in cellular innate immune response (1-4). When activated by double-stranded RNA, these proteins polymerize ATP into 2′-5′-linked oligomers (2,3,5). These 2'-5'-linked oligoadenylates bind to the ribonuclease RNase L, which then degrades viral and cellular RNA (2,3,5). In humans, the OAS family of proteins includes OAS1, OAS2, OAS3, and OASL (1-3). OAS3 is required to activate RNase L and to restrict the replication of four different human viruses (5). These findings suggest that OAS3 may provide a target for antiviral therapies (4,5). Alternate Names (2-5')oligo(A) synthase 3; (2-5')oligo(A) synthetase 3; 2-5A synthase 3; 2-5A synthetase 3; 2'-5'-oligoadenylate synthase 3; 2'-5'-oligoadenylate synthetase 3; 2'-5'-oligoadenylate synthetase 3, 100kDa; 2'-5'oligoadenylate synthetase p100; MGC133260; OAS3; p100; p100 OAS; p100OAS Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 120 SOURCE: Rabbit