CST, 41442S, FoxP3 (3G3) Mouse Monoclonal Antibody (APC Conjugate)

Monoclonal Antibody for studying FOXP3. Validated for Flow Cytometry (Fixed/Permeabilized). Highly specific and rigorously validated in-house, FoxP3 (3G3) Mouse Monoclonal Antibody (APC Conjugate) (CST #41442) is ready to ship. Product Usage Information For optimal flow cytometry results, we recommend 0.125μg of antibody per test. Flow Cytometry (Fixed/Permeabilized): 1:160 Storage Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.05% BSA, 0.05% NaN3, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. Protocol Available protocols: Flow Cytometry (Fixed/Permeabilized) Specificity / Sensitivity FoxP3 (3G3) Mouse Monoclonal Antibody (APC Conjugate) recognizes endogenous levels of total FoxP3 protein. This antibody conjugate detects an epitope within the intracellular domain. Species Reactivity: Mouse Source / Purification This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. Background Forkhead box (Fox) proteins are a family of evolutionarily conserved transcription factors containing a sequence known as Forkhead box or winged helix DNA binding domain (1). The human genome contains 43 Fox proteins that are divided into subfamilies. The FoxP subfamily has four members, FoxP1 - FoxP4, which are broadly expressed and play important roles in organ development, immune response, and cancer pathogenesis (2-4). The FoxP subfamily has several characteristics that are atypical among Fox proteins: their Forkhead domain is located at the carboxy-terminal region and they contain motifs that promote homo- and heterodimerization. FoxP proteins usually function as transcriptional repressors (4,5). FoxP3 is crucial for the development of T cells with regulatory properties (Treg) (6). Mutations in FoxP3 are associated with immune dysregulation, polyendocrinopathy, enteropathy, and X-linked syndrome (IPEX) (7), while overexpression in mice causes severe immunodeficiency (8). Research studies have shown that FoxP3 functions as a tumor suppressor in several types of cancer (9-11). Alternate Names AIID; DIETER; forkhead box P3; Forkhead box protein P3; Forkhead box protein P3 41 kDa form; Forkhead box protein P3, C-terminally processed; FOXP3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; IPEX; JM2; MGC141961; MGC141963; PIDX; Scurfin; XPID Specification REACTIVITY: M SENSITIVITY: Endogenous Source/Isotype: Mouse IgG1 kappa