CST, 4146S, Calpastatin Antibody

Polyclonal Antibody for studying calpastatin. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Calpastatin Antibody (CST #4146) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Calpastatin Antibody detects endogenous levels of total calpastatin protein. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human calpastatin. Antibodies are purified by peptide affinity chromatography. Background Calpain is a thiol proteinase that is functionally active as a heterodimer composed of a small regulatory subunit and one of at least two large catalytic subunits (calpain 1 or calpain 2). , calpain 1 (mu-calpain) requires micromolar levels of calcium, while calpain 2 (M-calpain) requires millimolar levels of calcium for activation (1). Calpastatin negatively regulates autoproteolytic cleavage of calpain 1 between Gly27 and Leu28 in a calcium dependent manner (2). In particular, calpastatin binds and inhibits calpain when calcium levels are high and is released when calcium levels go down. Calpastatin contains five domains, a unique N-terminal domain L with no inhibitory effects and four homologous domains (CAST 1-4) that can inhibit heterodimeric calpain 1 and 2 (3). Alternate Names BS-17; Calpain inhibitor; Calpastatin; CAST; heart-type calpastatin; ICAL; MGC9402; PLACK; Sperm BS-17 component Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 120 SOURCE: Rabbit