CST, 41490S, HAT1 (E7Y8Q) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HAT1. Validated for Western Blotting,Chromatin IP. Available in 2 sizes. Highly specific and rigorously validated in-house, HAT1 (E7Y8Q) Rabbit Monoclonal Antibody (CST #41490) is ready to ship. Product Usage Information For optimal ChIP results, use 5 μL of antibody and 10 μg of chromatin (approximately 4 × 10 6 cells) per IP. This antibody has been validated using SimpleChIP ® Enzymatic Chromatin IP Kits. Western Blotting: 1:1000 Chromatin IP: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Chromatin IP Specificity / Sensitivity HAT1 (E7Y8Q) Rabbit Monoclonal Antibody recognizes endogenous levels of total HAT1 protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val412 of human HAT1 protein. Background Histone acetyltransferase 1 (HAT1) is a member of the GCN5 family of acetyltransferases that can acetylate Lys5 and Lys12 on newly generated histone H4, a process that is essential for mammalian development (1-5). HAT1 binds to the H4 gene promoter, where it facilitates the production and acetylation of new histones. This process is strongly dependent on glucose availability, suggesting HAT1 plays a role in nutrient sensing (6). HAT1 has also been shown to contribute to recovery from replication-based DNA damage by incorporating H4Lys5/Lys12 acetylated histones at the sites of double-stranded breaks (7-9). HAT1 can acetylate non-histone proteins as well, and has been shown to play a key role in NF-kB signaling by acetylating PLZF, which in turn forms a complex with p50 to limit the production of cytokines (10). HAT1 overexpression has also been implicated in many cancer types (11-13). Alternate Names HAT1; histone acetyltransferase 1; Histone acetyltransferase type B catalytic subunit; KAT1 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 49 Source/Isotype: Rabbit IgG