CST, 41494T, APOBEC3B (E9A2G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying APOBEC3B. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, APOBEC3B (E9A2G) Rabbit Monoclonal Antibody (CST #41494) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity APOBEC3B (E9A2G) Rabbit Monoclonal Antibody recognizes endogenous levels of total APOBEC3B protein. This antibody weakly recognizes a 50 kDa band on unknown origen in cell lysates. Based on the sequence of the peptide antigen, this antibody is not expected to cross-react with other APOBEC3 family members. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro206 of human APOBEC3B protein. Background Members of the APOBEC3 subfamily of cytosine deaminases (APOBEC3A, APOBEC3B, APOBEC3C, APOBEC3D, APOBEC3F, APOBEC3G, and APOBEC3H) function in innate immunity by preventing viral DNA replication, including that of HIV-1 (1-2). APOBEC3B mutation and aberrant expression in cancer is thought to result in mutation of genomic DNA, and to drive tumorigenesis. APOBEC3B is highly expressed in human breast cancer, glioma, and other human cancers (3-5). Alternate Names A3B; ABC3B; APOBEC1L; APOBEC3B; apolipoprotein B mRNA editing enzyme catalytic subunit 3B; apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B; ARCD3; ARP4; bK150C2.2; cytidine deaminase; DJ742C19.2; DNA dC->dU-editing enzyme APOBEC-3B; FLJ21201; phorbolin 2; phorbolin 3; Phorbolin-1-related protein; Phorbolin-2/3; PHRBNL; Probable DNA dC->dU-editing enzyme APOBEC-3B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG