CST, 4155S, EPAC1 (5D3) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Epac1. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, EPAC1 (5D3) Mouse Monoclonal Antibody (CST #4155) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity EPAC1 (5D3) Mouse Monoclonal Antibody detects endogenous levels of total Epac1 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a recombinant fragment of human Epac1. Background EPAC1 and EPAC2 (exchange proteins activated by cyclic AMP) are guanine nucleotide exchange factors (GEFs) that catalyze the exchange of GDP for GTP, activating Rap1 and Rap2 small GTPases. Rap activation by EPAC is cAMP-dependent and mediates cAMP signaling in part through protein kinase A (PKA) (reviewed in 1). EPAC signaling plays a significant role in a number of cellular processes including migration and focal adhesion formation (2), exocytosis (3), insulin signaling (4), axon growth and guidance (5) and neurotransmitter release (6). Alternate Names 9330170P05Rik; bcm910; cAMP-GEFI; cAMP-regulated guanine nucleotide exchange factor I; CGEF1; EPAC; EPAC 1; EPAC1; Exchange factor directly activated by cAMP 1; Exchange protein directly activated by cAMP 1; HSU79275; MGC21410; Rap guanine nucleotide exchange factor (GEF) 3; Rap guanine nucleotide exchange factor 3; Rap1 guanine-nucleotide-exchange factor directly activated by cAMP; RAPGEF3; RPGF3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 100 Source/Isotype: Mouse IgG2a