CST, 41606S, UBASH3A (F7T5P) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying STS2. Validated for Western Blotting,Simple Western™,Immunoprecipitation. Available in 2 sizes. Highly specific and rigorously validated in-house, UBASH3A (F7T5P) Rabbit Monoclonal Antibody (CST #41606) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:50 - 1:250 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity UBASH3A (F7T5P) Rabbit Monoclonal Antibody recognizes endogenous levels of total UBASH3A protein. This antibody does not cross-react with UBASH3B protein and may detect bands of unknown origin around 25-30 kDa. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human UBASH3A protein. Background Ubiquitin-associated and SH3 domain-containing protein A (UBASH3A), also referred to as Suppressor of T cell receptor signaling 2 (STS-2), is an atypical protein tyrosine phosphatase that is primarily expressed in T cells (1). UBASH3A is a modular protein that contains an N-terminal UBA domain that can interact with ubiquitin, a central SH3 domain that mediates protein-protein interactions, and a C-terminal histidine phosphatase domain (1,2). Although it displays limited phosphatase activity compared to its homolog UBASH3B, UBASH3A functions as a negative regulator of T cell activation by downregulating the TCR-CD3 complex in a phosphatase-independent manner (3). UBASH3A also associates with the E3 ubiquitin ligase Cbl-b, which further dampens T cell activation through downregulation of CD28 (3). Due to its role in regulating T cell responses, UBASH3A dysfunction has been linked to several autoimmune disorders, including type 1 diabetes, rheumatoid arthritis, celiac disease, and systemic lupus erythematosus (4-7). Alternate Names Cbl-interacting protein 4; CLIP4; STS-2; STS2; Suppressor of T-cell receptor signaling 2; T-cell ubiquitin ligand; T-cell ubiquitin ligand 1; T-cell ubiquitin ligand protein; TULA; TULA-1; UBASH3A; ubiquitin associated and SH3 domain containing A; ubiquitin associated and SH3 domain containing, A; Ubiquitin-associated and SH3 domain-containing protein A; UBS3A Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 74, 80 Source/Isotype: Rabbit IgG