CST, 41653S, GAP43 (E6L2W) Mouse Monoclonal Antibody

Monoclonal Antibody for studying GAP43/Neuromodulin. Validated for WB,IHC,IF,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, GAP43 (E6L2W) Mouse Monoclonal Antibody (CST #41653) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Immunofluorescence (Frozen): 1:50 - 1:200 Immunofluorescence (Immunocytochemistry): 1:200 - 1:800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin), Immunofluorescence (Frozen), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity GAP43 (E6L2W) Mouse Monoclonal Antibody recognizes endogenous levels of total GAP43 protein. Non-specific staining of limited immune cells was observed by immunohistochemistry. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human GAP43 protein. Background GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's Disease (6,7). Alternate Names Axonal membrane protein GAP-43; B-50; calmodulin-binding protein P-57; GAP-43; GAP43; growth associated protein 43; Growth-associated protein 43; nerve growth-related peptide GAP43; NEUM; Neural phosphoprotein B-50; Neuromodulin; neuron growth-associated protein 43; pp46; protein F1 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 38, 43 Source/Isotype: Mouse IgG2b