CST, 4165S, Pim-3 (D17C9) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Pim-3. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Pim-3 (D17C9) Rabbit Monoclonal Antibody (CST #4165) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Pim-3 (D17C9) Rabbit Monoclonal Antibody detects endogenous levels of total Pim-3 protein. This antibody does not cross-react with other Pim family members. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser275 of human Pim-3. Background Pim proteins (Pim-1, Pim-2 and Pim-3) are oncogene-encoded serine/threonine kinases (1). Pim-1, a serine/threonine kinase highly expressed in hematopoietic cells, plays a critical role in the transduction of mitogenic signals and is rapidly induced by a variety of growth factors and cytokines (1-4). Pim-1 cooperates with c-Myc in lymphoid cell transformation and protects cells from growth factor withdrawal and genotoxic stress-induced apoptosis (5,6). Pim-1 also enhances the transcriptional activity of c-Myb through direct phosphorylation within the c-Myb DNA binding domain as well as phosphorylation of the transcriptional coactivator p100 (7,8). Hypermutations of the Pim-1 gene are found in B-cell diffuse large cell lymphomas (9). Phosphorylation of Pim-1 at Tyr218 by Etk occurs following IL-6 stimulation and correlates with an increase in Pim-1 activity (10). Various Pim substrates have been identified; Bad is phosphorylated by both Pim-1 and Pim-2 at Ser112 and this phosphorylation reverses Bad-induced cell apoptosis (11,12). Pim-3, originally named Kid-1, was identified as a protein induced by depolarization in PC-12 cells (13). Like other family members, Pim-3 can phosphorylate Bad to inhibit Bad-mediated apoptosis (14). Aberrant expression of Pim-3 has been observed in a number of types of cancer, including liver, pancreas, colon and gastric cancer (14-17). Alternate Names pim-3; pim-3 oncogene; Pim-3 proto-oncogene, serine/threonine kinase; PIM3; serine/threonine kinase Pim-3; serine/threonine protein kinase pim-3; Serine/threonine-protein kinase pim-3 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 35 Source/Isotype: Rabbit IgG