CST, 4167S, GGA3 Antibody

Polyclonal Antibody for studying GGA3. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, GGA3 Antibody (CST #4167) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity GGA3 Antibody detects endogenous levels of total GGA3 protein. This antibody can also detect a carboxy-terminal GGA3 fragment derived from caspase-mediated cleavage. This antibody may also cross-react with other proteins of unknown origin. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp520 of human GGA3. Antibodies are purified by protein A and peptide affinity chromatography. Background GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer's Disease (2). Alternate Names ADP-ribosylation factor-binding protein GGA3; GGA3; golgi associated, gamma adaptin ear containing, ARF binding protein 3; golgi-associated, gamma adaptin ear containing, ARF binding protein 3; Golgi-localized, gamma ear-containing, ARF-binding protein 3; KIAA0154 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 90 SOURCE: Rabbit