CST, 4174S, TPA (12-O-Tetradecanoylphorbol-13-Acetate)

Chemical Modulators for studying in the research area. Product Usage Information TPA is supplied as a 1 mg powder. Store at -20C. Before use, dissolve powder in 1 ml DMSO to yield a 1.6 mM stock solution. For working concentration of 200 μM, add 125 μl stock solution to 875 μl DMSO. Treat cells with 200 nM TPA for 20-30 minutes. TPA is soluble in DMSO and ethanol. Storage Store lyophilized or in solution at -20ºC, desiccated. Protect from light. In lyophilized form, the chemical is stable for 24 months. Once in solution, use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles. Background TPA (12-O-tetradecanoylphorbol-13-acetate) / PMA (phorbol-12-myristate-13-acetate) is the most commonly used phorbol ester. It binds and activates protein kinase C, causing a wide range of effects in cells and tissues (1,2). TPA has been demonstrated to be a potent tumor promoter in mouse skin (3). Conversely, it has been shown to be an effective cancer therapeutic agent in myelocytic leukemia patients, and has been indicated as a potential colorectal cancer therapeutic (4-5). TPA has also been shown to increase white blood cell and neutrophil counts in solid tumor cancer patients (6). Alternate Names phorbol ester; PKC; PMA; protein kinase C