CST, 41769SF, CDK6 (E3E3Q) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying Cdk6. Validated for Western Blotting,Immunohistochemistry (Paraffin). Highly specific and rigorously validated in-house, CDK6 (E3E3Q) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #41769) is ready to ship. Product Usage Information This product is the carrier free version of product #30483. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 30483 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity CDK6 (E3E3Q) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total CDK6 protein. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human CDK6 protein. Background The cyclin-dependent kinases form complexes with their cyclin partners and with CDK inhibitors. CDK6 and CDK4 associate with the D-type cyclins and target the retinoblastoma protein, allowing passage through the G1/S phase restriction point (1). CDK6/cyclin D complexes are sequestered in their inactive form through binding to one of the INK4 CDK inhibitor family members (2,3). Unlike the INK4 family of CDK inhibitors, the CDK inhibitor p21 Waf1/Cip1 may enhance the association of CDK4 and CDK6 with their cyclin D partners (4). Alternate Names CDK6; CDKN6; Cell division protein kinase 6; cyclin dependent kinase 6; Cyclin-dependent kinase 6; MCPH12; MGC59692; PLSTIRE; Serine/threonine-protein kinase PLSTIRE Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 36 Source/Isotype: Rabbit IgG