CST, 41892S, TCEB2/Elongin B (F3G5G) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TCEB2. Validated for Western Blotting,Simple Western™. Available in 2 sizes. Highly specific and rigorously validated in-house, TCEB2/Elongin B (F3G5G) Rabbit Monoclonal Antibody (CST #41892) is ready to ship. Product Usage Information Western Blotting: 1:1000 Simple Western™: 1:10 - 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TCEB2/Elongin B (F3G5G) Rabbit Monoclonal Antibody recognizes endogenous levels of total TCEB2/Elongin B protein. Based on sequence homology, this antibody is not predicted to cross-react with TCEB1/Elongin C protein. Species Reactivity: Human, Mouse, Rat, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp82 of human TCEB2/Elongin B protein. Background Transcription elongation factor B polypeptide 2 (TCEB2), also known as Elongin B, is a regulatory component of the SIII/Elongin elongation complex, which regulates elongation by RNA polymerase II by suppressing pausing of the polymerase during gene transcription. The SIII/Elongin complex is also composed of TCEB3/Elongin A, the catalytic subunit, and TCEB1/Elongin C, another regulatory subunit (1). TCEB2/Elongin B is part of the ubiquitin-proteasome pathway as a critical component of multiple E3 ligase complexes. It directly interacts with TCEB1/Elongin C as part of the VHL complex, which targets HIF-1α, a transcription factor that plays a critical role in the cellular response to hypoxia, for degradation (2,3). Dysregulation of HIF-α activity is associated with poor prognosis in multiple human cancers (4). TCEB2/Elongin B promotes therapy resistance in ovarian cancer (5,6), promotes breast cancer progression (7), and reduces radiosensitivity in triple-negative breast cancer (TNBC) (8). During HIV-1 infection, TCEB2/Elongin B is part of an E3 ligase complex that targets the anti-viral proteins APOBEC3F and APOBEC3G for degradation (9). Alternate Names EloB; Elongin 18 kDa subunit; elongin B; Elongin-B; elongin, 18-kD subunit; epididymis secretory sperm binding protein; RNA polymerase II transcription factor SIII p18 subunit; RNA polymerase II transcription factor SIII subunit B; SIII; SIII p18; TCEB2; transcription elongation factor B (SIII), polypeptide 2 (18kDa, elongin B); Transcription elongation factor B polypeptide 2; transcription elongation factor B subunit 2 Specification REACTIVITY: H M R Mk SENSITIVITY: Endogenous MW (kDa): 18 Source/Isotype: Rabbit IgG