CST, 41896T, TGF-beta Receptor II (E5M6F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying TGFBR2. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, TGF-beta Receptor II (E5M6F) Rabbit Monoclonal Antibody (CST #41896) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity TGF-beta Receptor II (E5M6F) Rabbit Monoclonal Antibody recognizes endogenous levels of total TGF-β Receptor II protein. This antibody does not cross-react with TGF-β Receptor I protein. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human TGF-β Receptor II protein. Background Transforming growth factor-β (TGF-β) proteins belong to the TGF-β superfamily of cytokines that play a critical role in regulating cell proliferation and differentiation, developmental patterning and morphogenesis, and disease pathogenesis (1-3). TGF-β ligands elicit signaling through three cell surface receptors: type I (RI), type II (RII), and type III (RIII) TGF-β receptors. Type I and type II receptors are serine/threonine kinases that form a heteromeric complex following ligand binding to the type II receptor. In response to ligand binding, the type II receptors form a stable complex with the type I receptors, triggering phosphorylation and activation of the type I receptor (4). This results in the recruitment of receptor-mediated SMADs (SMAD2, SMAD3), which are phosphorylated by the type I kinase in an SSXS domain in the C-terminus. This leads to recruitment of the co-SMAD (SMAD4), and subsequent translocation of this heteromeric SMAD complex to the nucleus, where it regulates transcription of target genes (5-7). The type III receptor, also known as betaglycan, is a transmembrane proteoglycan with a large extracellular domain that binds TGF-β with high affinity but lacks a cytoplasmic signaling domain. Expression of the type III receptor can regulate TGF-β signaling through presentation of the ligand to the signaling complex (8). Alternate Names AAT3; FAA3; LDS1B; LDS2; LDS2B; MFS2; RIIC; TAAD2; TbetaR-II; TBR-ii; TBRII; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta type II receptor; TGFbeta-RII; TGFBR2; TGFR-2; TGFR2; transforming growth factor beta receptor 2; transforming growth factor beta receptor II; transforming growth factor beta receptor type IIC; Transforming growth factor-beta receptor type II; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II alpha; transforming growth factor, beta receptor II beta; transforming growth factor, beta receptor II delta; transforming growth factor, beta receptor II epsilon; transforming growth factor, beta receptor II gamma Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 85 Source/Isotype: Rabbit IgG