CST, 4190S, Acetyl-CoA Carboxylase 1 Antibody

Polyclonal Antibody for studying acetyl-CoA carboxylase 1. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, Acetyl-CoA Carboxylase 1 Antibody (CST #4190) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity Acetyl-CoA Carboxylase 1 Antibody detects endogenous levels of total acetyl-CoA carboxylase 1 protein and does not cross-react with acetyl-CoA carboxylase 2. Immunofluorescence data indicate that the antibody is more reactive to rodent than human proteins. Species Reactivity: Human, Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human acetyl-CoA carboxylase 1 protein. Antibodies are purified by protein A and peptide affinity chromatography. Background Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA (1). It is the key enzyme in the biosynthesis and oxidation of fatty acids (1). In rodents, the 265 kDa ACC1 (ACCα) form is primarily expressed in lipogenic tissues, while 280 kDa ACC2 (ACCβ) is the main isoform in oxidative tissues (1,2). However, in humans, ACC2 is the predominant isoform in both lipogenic and oxidative tissues (1,2). Phosphorylation by AMPK at Ser79 or by PKA at Ser1200 inhibits the enzymatic activity of ACC (3). ACC is a potential target of anti-obesity drugs (4,5). Alternate Names ACAC; ACACA; ACACAD; ACC; ACC-alpha; ACC1; ACCA; Acetyl-CoA carboxylase 1; acetyl-CoA carboxylase alpha; acetyl-CoA carboxylase-alpha; acetyl-Coenzyme A carboxylase alpha; Biotin carboxylase Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 265 SOURCE: Rabbit