CST, 41910T, Spi-C (E7V7F) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying SPIC mouse. Validated for Western Blotting. Available in 2 sizes. Highly specific and rigorously validated in-house, Spi-C (E7V7F) Rabbit Monoclonal Antibody (CST #41910) is ready to ship. Product Usage Information Western Blotting: 1:1000 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting Specificity / Sensitivity Spi-C (E7V7F) Rabbit Monoclonal Antibody recognizes overexpressed levels of total Spi-C protein. This antibody detects a 70 kDa band of unknown origin in some cell lines. Species Reactivity: Mouse Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala205 of mouse Spi-C protein. Background Spi-C belongs to the erythroblast transformation-specific (ETS) domain family of transcription factors and binds to the purine-rich PU-box (1-3). Spi-C is a lineage-instructive factor involved in generating mature, specialized subsets of both myeloid and lymphoid cells (6). Spi-C is highly expressed by red pulp macrophages in the spleen, which are required for red blood cell recycling and iron homeostasis (4,6). Spi-C is also transiently expressed in B cells during specific stages of their development and regulates cell fate decisions at the pro-B to pre-B cell stage and plasma cell differentiation (1-3,6). Spi-C has also been shown to be a positive regulator that promotes osteoclast differentiation and function (5). Alternate Names AU019198; C76795; Prf; Pu.1-related factor; Spi-C; Spi-C transcription factor (Spi-1/PU.1 related); Spic; Transcription factor Spi-C Specification REACTIVITY: M SENSITIVITY: Transfected Only MW (kDa): 25, 28 Source/Isotype: Rabbit IgG