CST, 4191S, Phospho-Stathmin (Ser38) (D19H10) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying Stathmin (Ser38) phosphate. Validated for WB,IP,IHC,IF. Available in 2 sizes. Highly specific and rigorously validated in-house, Phospho-Stathmin (Ser38) (D19H10) Rabbit Monoclonal Antibody (CST #4191) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:200 Immunohistochemistry (Paraffin): 1:8000 Immunofluorescence (Immunocytochemistry): 1:12800 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. For a carrier free (BSA and azide free) version of this product see product # 85964 . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity Phospho-Stathmin (Ser38) (D19H10) Rabbit Monoclonal Antibody detects endogenous levels of stathmin protein only when phosphorylated at Ser38. Species Reactivity: Human, Monkey Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser38 of human stathmin protein. Background Stathmin is a ubiquitously expressed microtubule destabilizing phosphoprotein that is upregulated in a number of cancers. The amino terminus of the protein contains multiple phosphorylation sites and is involved in the promotion of tubulin filament depolymerization. Phosphorylation at these sites inactivates the protein and stabilizes microtubules. Ser16 phosphorylation by CaM kinases II and IV (1,2) increases during G2/M-phase and is involved in mitotic spindle regulation (3,4). Ser38 is a target for cdc2 kinase (5) and TNF-induced cell death gives rise to reactive oxygen intermediates leading to hyperphosphorylation of stathmin (6). EGF receptor activation of Rac and cdc42 also increases phosphorylation of stathmin on Ser16 and Ser38 (7). Other closely related family members are neuronally expressed and include SCG10, SCLIP, RB3 and its splice variants RB3' and RB3''. Stathmin and SCG10 have been shown to play roles in neuronal-like development in PC-12 cells (8). Alternate Names C1orf215; FLJ32206; Lag; LAP18; Leukemia-associated phosphoprotein p18; Metablastin; MGC138869; MGC138870; Oncoprotein 18; OP18; phosphoprotein 19; Phosphoprotein p19; pp17; pp19; PR22; Prosolin; Protein Pr22; SMN; Stathmin; stathmin 1; stathmin 1/oncoprotein 18; STMN1; testicular tissue protein Li 189; transmembrane protein C1orf215 Specification REACTIVITY: H Mk SENSITIVITY: Endogenous MW (kDa): 19, 20 Source/Isotype: Rabbit IgG