CST, 4194S, Sox1 Antibody

Polyclonal Antibody for studying SOX1. Validated for Western Blotting,Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, Sox1 Antibody (CST #4194) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunofluorescence (Frozen): 1:100 - 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunofluorescence (Frozen) Specificity / Sensitivity Sox1 Antibody detects endogenous levels of total Sox1 protein. This antibody does not cross-react with endogenous levels of other Sox proteins, including Sox2 and Sox3. Species Reactivity: Mouse, Rat Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues at the carboxy terminus of human Sox1. Antibodies are purified by Protein A and peptide affinity chromatography. Background (Sry-related box) genes encode a family of transcription factors that play a myriad of roles in developmental processes (1). Sox1 and Sox2 are the earliest markers that identify neuroectodermal tissue, and these markers, together with Sox9 collectively mark neuronal stem cells (NSC's) that are present in neurogenically active areas of the mature rodent brain (2). Sox1 has been found in the walls of the lateral ventricles and the dentate gyrus, as well as in inactive areas such as the cerebellum where it marks a population of cells in the Purkinje layer known as the Bergmann glia (2). Sox1 is thought to maintain the cell cycle and promote self renewal in NSCs, but also functions in other cell types to promote differentiation (3). Alternate Names SOX1; SRY (sex determining region Y)-box 1; SRY-box 1; SRY-box transcription factor 1; SRY-related HMG-box gene 1; Transcription factor SOX-1 Specification REACTIVITY: M R SENSITIVITY: Endogenous MW (kDa): 40 SOURCE: Rabbit