CST, 4196S, LIN28B Antibody

Polyclonal Antibody for studying Lin28B. Validated for Western Blotting,Immunoprecipitation. Highly specific and rigorously validated in-house, LIN28B Antibody (CST #4196) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunoprecipitation Specificity / Sensitivity LIN28B Antibody detects endogenous levels of total LIN28B protein. Species Reactivity: Human Source / Purification Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acid residues at the carboxyl terminus of human LIN28B. Antibodies are purified by Protein A and peptide affinity chromatography. Background LIN28A and LIN28B are conserved, developmentally regulated RNA binding proteins that inhibit the processing and maturation of the let-7 family of miRNAs (1,2). The let-7 miRNAs have been implicated in repression of oncogenes such as Ras, Myc, and HMGA2 (3). It has recently been shown that upregulation of LIN28A and LIN28B in primary human tumors and human cancer cell lines is correlated with downregulation of let-7 miRNAs (4). LIN28 genes are reported to be involved in primordial germ cell development and germ cell malignancy (5). In addition, allelic variation in LIN28B is associated with regulating the timing of puberty in humans (6). Overexpression of LIN28A, in conjunction with Oct-4, Sox2, and Nanog, can reprogram human fibroblasts to pluripotent, ES-like cells (7). Alternate Names CSDD2; FLJ16517; lin-28 homolog B; lin-28 homolog B (C. elegans); Lin-28.2; Lin-28B; LIN28B; LN28B; Protein lin-28 homolog B Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 32, 21 SOURCE: Rabbit