CST, 41997W2, Phospho-HS1 (Tyr378/397) (D12C1) Rabbit Monoclonal Antibody (SignalFlex™ Alexa Fluor® 750 Conjugate)

Monoclonal Antibody for studying HS1 (Tyr397) phosphate. Highly specific and rigorously validated in-house, Phospho-HS1 (Tyr378/397) (D12C1) Rabbit Monoclonal Antibody (SignalFlex^™ Alexa Fluor^® 750 Conjugate) (CST #41997) is ready to ship. Product Usage Information SignalFlex™ conjugates are produced using highly validated Cell Signaling Technology ® primary antibodies and conjugation methods that have been rigorously tested, ensuring high-quality conjugates and lot-to-lot consistency. These conjugates are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. However, they are not tested on specific assays. Optimal dilutions/concentrations should be determined by the end user. When performing flow cytometry, we recommend using an isotype control conjugate at the same concentration as the antibody conjugate. Storage Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. Specificity / Sensitivity Phospho-HS1 (Tyr378/397) (D12C1) XP Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr405 of mouse HS1 protein. This site corresponds to Tyr397 of human HS1 protein. Background HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7). HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10). Alternate Names cortactin-like; CTTNL; HCLS1; Hematopoietic cell-specific LYN substrate 1; Hematopoietic lineage cell-specific protein; HS1; LckBP1; p75 Specification REACTIVITY: H SENSITIVITY: Endogenous Source/Isotype: Rabbit IgG