CST, 42105S, HDGF (E3P7K) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying HDGF. Validated for Western Blotting,Immunoprecipitation,Immunofluorescence (Immunocytochemistry). Available in 2 sizes. Highly specific and rigorously validated in-house, HDGF (E3P7K) Rabbit Monoclonal Antibody (CST #42105) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunoprecipitation: 1:50 Immunofluorescence (Immunocytochemistry): 1:400 - 1:1600 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody . Protocol Available protocols: Western Blotting, Immunoprecipitation, Immunofluorescence (Immunocytochemistry) Specificity / Sensitivity HDGF (E3P7K) Rabbit Monoclonal Antibody recognizes endogenous levels of total HDGF protein. Based on the amino acid sequence of the antigenic peptide, this antibody is expected to recognize isoforms 1-3 of HDGF (HDGF-A, -B and -C). Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala229 of human HDGF protein. Background HDGF (hepatoma-derived growth factor) acts as both a transcription factor and a secreted protein to regulate multiple cellular functions such as transformation, apoptosis, angiogenesis, proliferation, and metastasis. HDGF is involved in the progression of multiple human cancers, and its elevated expression is a negative prognostic marker in cancer progression (1,2). Alternate Names DKFZp686J1764; epididymis secretory sperm binding protein; FLJ96580; HDGF; heparin binding growth factor; hepatoma derived growth factor; Hepatoma-derived growth factor; High mobility group protein 1-like 2; high-mobility group protein 1-like; HMG-1L2; HMG1L2 Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 40-45 Source/Isotype: Rabbit IgG