CST, 42120SF, TBR1 (D6C6X) Rabbit Monoclonal Antibody (BSA and Azide Free)

Monoclonal Antibody for studying TBR1. Validated for Immunohistochemistry (Paraffin),Immunofluorescence (Frozen). Highly specific and rigorously validated in-house, TBR1 (D6C6X) Rabbit Monoclonal Antibody (BSA and Azide Free) (CST #42120) is ready to ship. Product Usage Information This product is the carrier free version of product #49661. All data were generated using the same antibody clone in the standard formulation which contains BSA and glycerol. This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us . Optimal dilutions/concentrations should be determined by the end user. BSA and Azide Free antibodies are quality control tested by size exclusion chromatography (SEC) to determine antibody integrity. Formulation Supplied in 1X PBS (10 mM Na 2 HPO 4 , 3 mM KCl, 2 mM KH 2 PO 4 , and 140 mM NaCl (pH 7.8)). BSA and Azide Free. For standard formulation of this product see product # 49661 Storage Store at -20°C. This product will freeze at -20°C so it is recommended to aliquot into single-use vials to avoid multiple freeze/thaw cycles. A slight precipitate may be present and can be dissolved by gently vortexing. This will not interfere with antibody performance. Specificity / Sensitivity TBR1 (D6C6X) Rabbit Monoclonal Antibody (BSA and Azide Free) recognizes endogenous levels of total TBR1 protein. Low levels of nuclear staining of unknown specificity have been observed in mouse small intestine, spleen, and pancreas. Species Reactivity: Human, Mouse, Rat Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro415 of human TBR1 protein. Background T-box, brain, 1 (TBR1) is a transcription factor important in vertebrate embryo development. As a member of T-Box family of transcription factors, TBR1 is expressed in postmitotic glutamatergic projection neurons (1). During cortical neurogenesis, sequential expression of transcription factors Pax6, TBR2, and TBR1 regulates discrete steps in projection neuron differentiation (2). TBR1 is enriched in layer 6 of the developing cortex. In the absence of TBR1, mutants exhibit profound defects in frontal cortex and layer 6 differentiation, suggesting that TBR1 regulates regional and laminar identity of postmitotic cortical neurons (3). Therefore, TBR1 expression can be used as a marker for postmitotic glutamatergic neurons and cortical laminar specificity. T-box, brain, 1 (TBR1) is a transcription factor important in vertebrate embryo development. As a member of T-Box family of transcription factors, TBR1 is expressed in postmitotic glutamatergic projection neurons (1). During cortical neurogenesis, sequential expression of transcription factors Pax6, TBR2, and TBR1 regulates discrete steps in projection neuron differentiation (2). TBR1 is enriched in layer 6 of the developing cortex. In the absence of TBR1, mutants exhibit profound defects in frontal cortex and layer 6 differentiation, suggesting that TBR1 regulates regional and laminar identity of postmitotic cortical neurons (3). Therefore, TBR1 expression can be used as a marker for postmitotic glutamatergic neurons and cortical laminar specificity. Alternate Names IDDAS; MGC141978; T-box brain protein 1; T-box brain transcription factor 1; T-box, brain 1; T-box, brain, 1; T-brain-1; TBR-1; TBR1; TES-56 Specification REACTIVITY: H M R SENSITIVITY: Endogenous MW (kDa): 74 Source/Isotype: Rabbit IgG