CST, 42153S, KISS1 (E6H9S) Rabbit Monoclonal Antibody

Monoclonal Antibody for studying KiSS1. Validated for Western Blotting,Immunohistochemistry (Paraffin). Available in 2 sizes. Highly specific and rigorously validated in-house, KISS1 (E6H9S) Rabbit Monoclonal Antibody (CST #42153) is ready to ship. Product Usage Information Western Blotting: 1:1000 Immunohistochemistry (Paraffin): 1:100 - 1:400 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Immunohistochemistry (Paraffin) Specificity / Sensitivity KISS1 (E6H9S) Rabbit Monoclonal Antibody recognizes endogenous levels of total KISS1 protein. This antibody detects a band of unknown origin at 35 kDa by western blot. Non-specific staining was observed in pancreatic acinar cells by immunohistochemistry. Species Reactivity: Human Source / Purification Monoclonal antibody is produced by immunizing animals with recombinant protein specific to human KISS1 protein. Background The KiSS-1 receptor (KISS1R, GPR54) is a G protein-coupled receptor that inhibits cancer cell metastasis and plays a major role in gonadotropic axis physiology (1). The GPR54 protein was originally described as an orphan receptor homologous to the galanin receptor, and later identified as a receptor for amidated peptide products of the metastasis suppressor gene ( , Kisspeptin-1) (2,3). In humans, amidated kisspeptin ligands are produced predominantly in cells of the arcuate nucleus and preoptic area, with expression controlled by gonadal hormones (4). Research studies show that deletion of either the KiSS-1 receptor or gene leads to hypogonadotropic hypogonadism, a disorder characterized by reduced levels of circulating testosterone and gonadotropins, as well as abnormal sexual maturation (5,6). The administration of kisspeptins potently stimulates gonadotropin secretion, indicating that KISS1R and kisspeptins play a major role in the physiology of the gonadotropic axis (7). Additional research demonstrates that KISS1R and kisspeptins inhibit metastasis in cancer cells by inhibiting cell motility (8). However, other studies indicate that increased expression of KISS1R and its ligands in human breast tumors correlates with higher tumor grade and metastatic potential, likely by engaging MMP-9 activation via transactivation of EGFR (9). Alternate Names HH13; KiSS-1; KiSS-1 metastasis suppressor; KiSS-1 metastasis-suppressor; KISS1; kisspeptin; Kisspeptin-1; Kisspeptin-10; Kisspeptin-13; Kisspeptin-14; Kisspeptin-54; malignant melanoma metastasis-suppressor; Metastasis-suppressor KiSS-1; Metastin; MGC39258; prepro-kisspeptin Specification REACTIVITY: H SENSITIVITY: Endogenous MW (kDa): 14 Source/Isotype: Rabbit IgG