CST, 42172S, SARS-CoV-2 Spike Protein (S1-NTD) (E7M5X) Mouse Monoclonal Antibody

Monoclonal Antibody for studying Spike Protein SARS-CoV-2. Validated for Western Blotting,Flow Cytometry (Live). Available in 2 sizes. Highly specific and rigorously validated in-house, SARS-CoV-2 Spike Protein (S1-NTD) (E7M5X) Mouse Monoclonal Antibody (CST #42172) is ready to ship. Product Usage Information Western Blotting: 1:1000 Flow Cytometry (Live): 1:200 Storage Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at -20°C. Do not aliquot the antibody. Protocol Available protocols: Western Blotting, Flow Cytometry (Live) Specificity / Sensitivity SARS-CoV-2 Spike Protein (S1-NTD) (E7M5X) Mouse Monoclonal Antibody recognizes endogenous levels of total SARS-CoV-2 spike protein. This antibody detects full-length protein, and also detects the S1 fragment generated by furin cleavage. This antibody does not cross-react with spike proteins from SARS or MERS coronaviruses. Species Reactivity: Virus Source / Purification Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro25 of SARS-CoV-2 spike protein. Background The cause of the COVID-19 pandemic is a novel and highly pathogenic coronavirus, termed SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2). SARS-CoV-2 is a member of the Coronaviridae family of viruses (1). The genome of SARS-CoV-2 is similar to other coronaviruses, and is comprised of four key structural proteins: S, the spike protein, E, the envelope protein, M, the membrane protein, and N, the nucleocapsid protein (2). Coronavirus spike proteins are class I fusion proteins and harbor an ectodomain, a transmembrane domain, and an intracellular tail (3,4). The highly glycosylated ectodomain projects from the viral envelope surface and facilitates attachment and fusion with the host cell plasma membrane. The ectodomain can be further subdivided into host receptor-binding domain (RBD) (S1) and membrane-fusion (S2) subunits, which are produced upon proteolysis by host proteases at S1/S2 and S2' sites. S1 and S2 subunits remain associated after cleavage and assemble into crown-like homotrimers (2,4). In humans, both SARS-CoV and SARS-CoV-2 spike proteins utilize the angiotensin-converting enzyme 2 (ACE2) protein as a receptor for cellular entry (5-7). Spike protein subunits represent a key antigenic feature of coronavirus virions, and therefore represent an important target of vaccines, novel therapeutic antibodies, and small-molecule inhibitors (8,9). Alternate Names 2019-nCoV surface glycoprotein; Peplomer protein; S glycoprotein; SARS-CoV-2 spike protein; spike glycoprotein; SPIKE_SARS2; surface glycoprotein Specification REACTIVITY: Vir SENSITIVITY: Endogenous MW (kDa): 110, 220 Source/Isotype: Mouse IgG1